A strong suit of MIKAIA® is its wide portfolio of analysis apps for multiplexed immunofluorescence (mIF) / spatial proteomics. It can be used for both low-plex and high-plex panels and is compatbile with image formats...
Tag - Use Case
MIKAIA University Use Case
Volker Bruns
Analyzing MACSima 47-plex mIF with MIKAIA: AI Cell Segmentation + Cell Typing + Cell-cell Connections + Cellular Neighborhoods
This MIKAIA® app note demonstrates how to analyze a 47-plex spatial proteomics scan created with MACSima ™ (by Miltenyi Biotec), utilizing sequential immunofluorescence for the analysis: Instrument: MACSima™ by Miltenyi...
High Content Imaging: Analyzing Ex-Vivo Drug Response Assays with MIKAIA
Researchers at the Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM) have developed PEDRA: Platform for Ex-Vivo Drug Response Assays. Up to 100 compounds are tested in parallel on primary patient...
Proximity Analysis: Quantifying Cell Subpopulations in Vicinity of Intratumoral Microdevice (IMD)
The Laboratory for Bio-Micro Devices at Brigham and Women’s Hospital () develops drug releasing implantable intratumoral microdevices (IMD) [1]. Using MIKAIA®, they want to examine in a quantitative fashion how...
Programming-free Single Cell Analysis of CODEX TMA with MIKAIA
CODEX, now called PhenoCycler, by Akoya Biosciences, along with other sequential immunofluorescence technologies (e.g., Lunaphore (Comet), Miltenyi Biotech (MACSima), Leica Microsystems (Cell DIVE), or other vendors)...
Quantify Histological Growth Pattern or Spatial Heterogeneity with the Grid Analysis App
Result of Grid Analysis App shows histological growth pattern and spatial heterogeneity
MIKAIA: Differential IHC Cell Detection by ROI
This article is based on a our presentation “IHC Cell Analysis — More than just Cell Counting: A proposed Workflow” at the European Conference for Digital Pathology (ECDP) 2024 in Vilnius, Lithuania...
MIKAIA: Quantifying Perineural Invasion in Duplex IHC
This MIKAIA® App Note shows how to quantify tumor in proximity to nerve fibers (perineural invasion) in a IHC duplex-stained tissue section, where nerves appear red (antigen: S100) and tumor brown (antigen:...
IHC Profiler in MIKAIA – Assessing New IHC Assay Using Multi-organ TMA
When designing a new immunohistochemistry (IHC) assay, a quantitative assessment of how it “responds” in different tissues of various organs is insightful and helps optimizing the assay in an objective way...
MIKAIA-Analysis of Human Pancreas 4plex Scanned with NanoString GeoMx DSP
In this MIKAIA® University application note, we guide you through the analysis of a common use case, coexpression of marker combinations in islets of Langerhans and surrounding tissue in a pancreas 4plex imaged with...
Nathalie Falk