Volker BrunsNew to MIKAIA®? This blog post will hopefully help you take the first steps.
Introduction video (10 min)
Important concepts
It is helpful to be aware of these concepts when working with MIKAIA®:
Where to get help?
The 100+ pages user manual is the most detailed resource. It is available from within MIKAIA®, by clicking in the main menu on “Help” and then “Open Manual”. The pdf is located in the MIKAIA® installation folder, and is available for download here: Download MIKAIA® Manual
The FAQs article contains a collection of important bits and pieces: FAQs
You have not found an answer to your question or want to discuss your project? You want to schedule a personal MIKAIA® training? Reach out at mikaia@iis.fraunhofer.de. We are here to help.
Image analysis
If you want to analyze fluorescence scans, regardless of 2plex, 20plex or 50plex, this article will give you a good overview about the typical analysis steps such as cell segmentation, cell typing and spatial analyses:
- Overview of MIKAIA’s Spatial Analysis Apps for Multiplexed Immunofluorescence Slides
- mIF Cell AI Gallery
Another widels used app is the Universal IHC Cell Detection AI:
- IHC Cell Detection App explained (23min video tutorial, v2.4, Oct 2025)
- IHC Cell Detection App Immune cell infiltration in tumor micro environment (TME) (June 2023)
- IHC Cell Quantification AI Gallery
An introduction to using the AI Author App for analyzing your brightfield H&E or IHC scans is available here:
Evaluating MIKAIA
Contact mikaia@iis.fraunhofer.de and ask for a set of one-time voucher codes that let you try out the Apps locked in MIKAIA lite.
Refer to this article for help on how to import the voucher codes: link.
Activating MIKAIA after purchase
After a purchase you create a license request file that contains your computer’s unique fingerprint. Based on that, the activation file will be generated and returned to you. Refer to this article for a step by step guide: Getting Started: Activating MIKAIA
Grit Nickel
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