{"id":4263,"date":"2025-07-15T09:50:03","date_gmt":"2025-07-15T07:50:03","guid":{"rendered":"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/?p=4263"},"modified":"2025-10-14T10:56:28","modified_gmt":"2025-10-14T08:56:28","slug":"ihc-cell-quantification-ai-gallery","status":"publish","type":"post","link":"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/ihc-cell-quantification-ai-gallery\/","title":{"rendered":"MIKAIA\u00a0IHC Cell Quantification AI Gallery"},"content":{"rendered":"\n\n\n\n<p>This gallery illustrates for a range of different tissue types and IHC markers the cell analysis quality of <a href=\"http:\/\/www.mikaia.ai\">MIKAIA<\/a>&#8216;s IHC Cell Detection App. The app first unmixes the stain components, then uses an AI to delineate cells, then computes the stains&#8217; intensity per cell and finally classifies the cells into negative \/ positive (or low \/ medium \/ strong positive) based on the marker intensity.<\/p>\n\n\n\n<p>Cells with a too low intensity, too small or large size, black color, or cells that are located in an &#8220;ignore&#8221; annotations can optionally be filtered out. Cells can also be grouped by ROI, e.g., the <a href=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/digital-pathology-batch-analysis-with-mikaia\/\">AI Author App<\/a> can be used to recognize tumor vs non-tumor areas. The <a href=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/ihc-cell-detection-by-roi\/\">IHC Cell Detection App<\/a> can then either be used to only analyze cells in the previously detected tumor region or it can analyze cells everywhere, but separate cell types based on what ROI (e.g., tumor or non-tumor) they are located in.<\/p>\n\n\n\n<p>The IHC Cell Detection App also includes post-processing functionality, e.g., it can search hotspots, group positive cells into clusters, or create a heatmap. Additional apps offer further functionality, e.g., the <a href=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/proximity-analysis-imd\/\">Proximity Analysis App<\/a> can be used to compute distances from cells to the tumor interface or between cell types.<\/p>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4579.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4579.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4579.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4579.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4579  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"1\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/09\/IHC-Human-Breast-Her2-subcellular-without-markup.png\" alt=\"Human Breast Her2 subcellular\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/09\/IHC-Human-Breast-Her2-subcellular-with-markup.png\" alt=\"Human Breast Her2 subcellular with markup\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4579 bafg-slider-info\">\n\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4273.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4273.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4273.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4273.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4273  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/Gallery-IHC-CD3-Mousebrain-1-scaled.jpg\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/Gallery-IHC-CD3-Mousebrain-2-scaled.jpg\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4273 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">CD3 Mouse brain<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4314.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4314.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4314.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4314.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4314  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/Gallery-IHC-B220-Mousecolon-1-scaled.jpg\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/Gallery-IHC-B220-Mousecolon-2-scaled.jpg\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4314 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">B220 Mouse Colon<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4315.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4315.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4315.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4315.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4315  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Mouse-Colon-B220-02-without-markup.png\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Mouse-Colon-B220-02-with-markup.png\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4315 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">B220 Mouse Colon (ROI 2)<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4319.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4319.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4319.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4319.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4319  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Mouse-Brain-IBA1-without-markup.png\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Mouse-Brain-IBA1-with-markup.png\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4319 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">IBA1 Mouse Brain<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4353.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4353.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4353.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4353.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4353  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Mouse-Brain-CK8-without-markup.png\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Mouse-Brain-CK8-with-markup.png\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4353 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">CK8 Mouse Brain<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4356.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4356.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4356.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4356.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4356  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Tongue-Ki67-fov1-without-markup.png\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Tongue-Ki67-fov1-with-markup.png\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4356 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">Ki67 Human Tongue<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4361.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4361.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4361.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4361.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4361  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Tongue-CD45-fov1-without-markup.png\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Tongue-CD45-fov1-with-markup.png\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4361 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">CD45 Human Tongue (ROI 1)<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4360.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4360.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4360.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4360.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4360  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Tongue-CD45-fov2-without-markup.png\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Tongue-CD45-fov2-with-markup.png\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4360 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">CD45 Human Tongue (ROI 2)<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4364.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4364.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4364.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4364.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4364  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Colon-CD3-fov1-without-markup.png\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Colon-CD3-fov1-with-markup.png\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4364 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">CD3 Human Colon<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4366.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4366.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4366.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4366.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4366  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Breast-PGR-overview-without-markup.png\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Breast-PGR-overview-with-markup.png\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4366 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">PGR Human Breast (overview)<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4367.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4367.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4367.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4367.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4367  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Breast-PGR-fov1-without-markup.png\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Breast-PGR-fov1-with-markup.png\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4367 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">PGR Human Breast (ROI 1)<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4368.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4368.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4368.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4368.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4368  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Breast-Ki67-without-markup.png\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Breast-Ki67-with-markup.png\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4368 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">Ki67 Human Breast<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4369.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4369.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4369.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4369.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4369  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" bafg-overlay=\"\"\n\t\t\t\tbafg-move-slider-on-hover=\"\"\n\t\t\t\tbafg-click-to-move=\"\">\n\n\t\t\t\t\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Breast-Her2-overview-without-markup.png\" alt=\"\">\n\t\t\t\t<img class=\"skip-lazy\" data-skip-lazy\t\t\t\t\tsrc=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/IHC-Human-Breast-Her2-overview-with-markup.png\" alt=\"\">\n\n\t\t\t<\/div>\n\n\t\t\t\t<div class=\"bafg-slider-info-wraper\">\n\t\t<div style=\"\" class=\"slider-info-4369 bafg-slider-info\">\n\t\t\t\t\t\t\t<h2 class=\"bafg-slider-title\">HER2 Human Breast (overview)<\/h2>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n\t\n\t\t\t<style type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4372.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4372.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4372.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: 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type=\"text\/css\">\n\t\t\t\t\t\t\t\t\t\t\t<\/style>\n\t\t\t\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\t\t\t\n\t<style type=\"text\/css\">\n\t\t.slider-info-4370.bafg-slider-info .bafg-slider-title {\n\t\t\t\t\t\t\tfont-size:\n\t\t\t\t\t22px\t\t\t\t;\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4370.bafg-slider-info .bafg-slider-description {\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t\t\n\t\t\n\t\t.slider-info-4370.bafg-slider-info .bafg_slider_readmore_button {\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\ttext-align: center;\n\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\n\t\t.slider-info-4370.bafg-slider-info .bafg_slider_readmore_button:hover {\n\n\t\t\t\n\t\t\t\n\t\t\t\t\t}\n\t<\/style>\n\t\n\t\t\t<div class=\"bafg-twentytwenty-container slider-4370  \"\n\t\t\t\tbafg-orientation=\"horizontal\" bafg-default-offset=\"0.5\"\n\t\t\t\tbafg-before-label=\"Before\"\n\t\t\t\tbafg-after-label=\"After\" 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analysis quality of MIKAIA&#8216;s IHC Cell Detection App. The app first unmixes the stain components, then uses an AI to delineate cells, then computes the stains&#8217; intensity per cell and finally classifies the cells into negative \/ positive (or low [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":4391,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3,35,28],"tags":[37,107,87,7,29],"coauthors":[56],"class_list":["post-4263","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-digital-pathology","category-life-science","category-mikaia-university","tag-ai","tag-gallery","tag-ihc","tag-mikaia","tag-mikaia-app-note"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>MIKAIA\u00a0IHC Cell Quantification AI Gallery<\/title>\n<meta name=\"description\" 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insights\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/FraunhoferIIS\" \/>\n<meta property=\"article:published_time\" content=\"2025-07-15T07:50:03+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-10-14T08:56:28+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2025\/07\/banner.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1861\" \/>\n\t<meta property=\"og:image:height\" content=\"782\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Volker Bruns\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Volker Bruns\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script 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