{"id":2354,"date":"2024-07-30T04:16:35","date_gmt":"2024-07-30T02:16:35","guid":{"rendered":"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/?p=2354"},"modified":"2025-10-29T21:57:09","modified_gmt":"2025-10-29T20:57:09","slug":"2-articles-in-trillium-pathology-2024","status":"publish","type":"post","link":"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/2-articles-in-trillium-pathology-2024\/","title":{"rendered":"2 Articles in Trillium Pathology 2024"},"content":{"rendered":"\n<p>We have contributed two article&#8217;s to this year&#8217;s Trillium Pathology issue. Here are the abstracts, with links to the full articles:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>V. Bruns, <strong>Programming-free Image-Analysis Workflows for IHC, IF and Spatial Proteomics: More than just Cell Counting<\/strong><\/li>\n\n\n\n<li>V. Bruns &amp; C.-A. Weis, <strong>A growing number of AIs cleared for clinical use is finally available: The AI-assisted Pathologist<\/strong><\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h1 class=\"wp-block-heading\">Programming-free Image-Analysis Workflows for IHC, IF, and Spatial Proteomics: More than just Cell Counting<\/h1>\n\n\n\n<p><strong>Volker Bruns<\/strong>, DOI:&nbsp;<a href=\"https:\/\/doi.org\/10.47184\/tp.2024.01.06\">https:\/\/doi.org\/10.47184\/tp.2024.01.06<\/a><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"576\" src=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-3-1024x576.png\" alt=\"\" class=\"wp-image-2356\" srcset=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-3-1024x576.png 1024w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-3-300x169.png 300w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-3-768x432.png 768w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-3-370x208.png 370w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-3-270x152.png 270w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-3-570x321.png 570w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-3-740x416.png 740w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-3.png 1200w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>Quantitative analysis of cells using a specific protein marker is one of the most frequently performed tasks in both preclinical and clinical histopathology. The primary alternatives include immunohistochemistry and immunofluorescence. This article will briefly shed some light on the pros and cons of both methods, the main ones being that immunohistochemistry is widely available, but in many laboratories, it is limited to only one marker per image. <\/p>\n\n\n\n<p>Utilizing more than one marker can be achieved through methods such as establishing duplex or triplex stains, serial sections, or re-staining. However, immunofluorescence excels where a deep multi-marker characterization of single cells is required. Spatial proteomics systems have recently increased the plexity to as many as 100 parallel markers, allowing advanced co-expression, rare cells, and spatial neighbourhood analyses. Bioinformatic analysis aspects for both modalities are discussed, outlining two generic workflows as they are realised in a professional image analysis software used by biomedical researchers. While cell segmentation and typing are at the core, a number of pre- and post-processing steps, such as tissue detection, comparison of ROIs, hotspot search, spatial clustering or neighbourhood analysis should be performed to provide more comprehensive read-outs.<\/p>\n\n\n\n<p><strong>Keywords:<\/strong>&nbsp;Digital pathology, spatial proteomics, image analysis, IHC, IF<\/p>\n\n\n\n<p><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h1 class=\"wp-block-heading\">A growing number of AIs cleared for clinical use is finally available: The AI-assisted Pathologist<\/h1>\n\n\n\n<p><strong>Volker Bruns &amp; Cleo-Aron Weis<\/strong>, DOI:&nbsp;<a href=\"https:\/\/doi.org\/10.47184\/tp.2024.01.03\">https:\/\/doi.org\/10.47184\/tp.2024.01.03<\/a><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"511\" src=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-4-1024x511.png\" alt=\"\" class=\"wp-image-2357\" srcset=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-4-1024x511.png 1024w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-4-300x150.png 300w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-4-768x383.png 768w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-4-370x185.png 370w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-4-270x135.png 270w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-4-570x285.png 570w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-4-740x369.png 740w, https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/image-4.png 1200w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>As more pathology laboratories are transitioning to a digital workflow, the availability of commercial Artificial Intelligence assistance systems is also increasing. Today nearly 40 such products approved for diagnostic use are available. This article provides an overview of the most widely addressed use cases, including Immunohistochemistry scoring for breast cancer and non-small-cell lung cancer, Gleason grading for prostate cancer, or metastasis detection in lymph nodes. <\/p>\n\n\n\n<p>While automation alone already promises an increase in efficiency that may help to bridge the growing gap between supply (pathology work force) and demand (histological testing), this article introduces another category of Artificial Intelligence products that go beyond just mimicking today\u2019s established score. Various Artificial Intelligence are being introduced that detect genetic alterations or stratify risk, directly from the standard hematoxylin &amp; eosin staining. Finally, a brief outlook explains how basic AI models are currently finding their way into computational pathology and promise to further accelerate product developments by decreasing the time-to-model.<\/p>\n\n\n\n<p><strong>Keywords:<\/strong>&nbsp;AI, digital pathology, CE-IVDR, FDA, foundation models<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p>Copyright: Fraunhofer IIS<\/p>\n","protected":false},"excerpt":{"rendered":"<p>We have contributed two article&#8217;s to this year&#8217;s Trillium Pathology issue. Here are the abstracts, with links to the full articles: Programming-free Image-Analysis Workflows for IHC, IF, and Spatial Proteomics: More than just Cell Counting Volker Bruns, DOI:&nbsp;https:\/\/doi.org\/10.47184\/tp.2024.01.06 Quantitative analysis of cells using a specific protein marker is one of the most frequently performed tasks [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":2359,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3,35,24],"tags":[37,7,30],"coauthors":[56],"class_list":["post-2354","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-digital-pathology","category-life-science","category-spatial-biology","tag-ai","tag-mikaia","tag-publication"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>2 Articles in Trillium Pathology 2024 - SMART SENSING insights<\/title>\n<meta name=\"description\" content=\"We have contributed 2 article&#039;s to this year&#039;s Trillium Pathology issue: &#039;More than just Cell Counting&#039; and &#039;The AI-assisted Pathologist&#039;.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/2-articles-in-trillium-pathology-2024\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"2 Articles in Trillium Pathology 2024 - SMART SENSING insights\" \/>\n<meta property=\"og:description\" content=\"We have contributed 2 article&#039;s to this year&#039;s Trillium Pathology issue: &#039;More than just Cell Counting&#039; and &#039;The AI-assisted Pathologist&#039;.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/2-articles-in-trillium-pathology-2024\/\" \/>\n<meta property=\"og:site_name\" content=\"SMART SENSING insights\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/FraunhoferIIS\" \/>\n<meta property=\"article:published_time\" content=\"2024-07-30T02:16:35+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-10-29T20:57:09+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/websites.fraunhofer.de\/smart-sensing-insights\/wp-content\/uploads\/2024\/07\/Trillium-Pathology_Titelbild_24_be37829752-wide.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1916\" \/>\n\t<meta property=\"og:image:height\" content=\"1010\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Volker Bruns\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Volker Bruns\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/websites.fraunhofer.de\\\/smart-sensing-insights\\\/2-articles-in-trillium-pathology-2024\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/websites.fraunhofer.de\\\/smart-sensing-insights\\\/2-articles-in-trillium-pathology-2024\\\/\"},\"author\":{\"name\":\"Volker Bruns\",\"@id\":\"https:\\\/\\\/websites.fraunhofer.de\\\/smart-sensing-insights\\\/#\\\/schema\\\/person\\\/fd37d35c4d3576840cf0bc3f74eafb98\"},\"headline\":\"2 Articles in Trillium Pathology 2024\",\"datePublished\":\"2024-07-30T02:16:35+00:00\",\"dateModified\":\"2025-10-29T20:57:09+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/websites.fraunhofer.de\\\/smart-sensing-insights\\\/2-articles-in-trillium-pathology-2024\\\/\"},\"wordCount\":479,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\\\/\\\/websites.fraunhofer.de\\\/smart-sensing-insights\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/websites.fraunhofer.de\\\/smart-sensing-insights\\\/2-articles-in-trillium-pathology-2024\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/websites.fraunhofer.de\\\/smart-sensing-insights\\\/wp-content\\\/uploads\\\/2024\\\/07\\\/Trillium-Pathology_Titelbild_24_be37829752-wide.jpg\",\"keywords\":[\"AI\",\"MIKAIA\u00ae\",\"Publication\"],\"articleSection\":[\"Digital Pathology\",\"Life Science\",\"Spatial Biology\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/websites.fraunhofer.de\\\/smart-sensing-insights\\\/2-articles-in-trillium-pathology-2024\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/websites.fraunhofer.de\\\/smart-sensing-insights\\\/2-articles-in-trillium-pathology-2024\\\/\",\"url\":\"https:\\\/\\\/websites.fraunhofer.de\\\/smart-sensing-insights\\\/2-articles-in-trillium-pathology-2024\\\/\",\"name\":\"2 Articles in Trillium Pathology 2024 - 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